大孔吸附树脂一次性分离杜仲叶中杜仲总苷和杜仲黄酮的研究

董娟娥; 梁宗锁; 张康健; 张利萍; 李倩

大孔吸附树脂一次性分离杜仲叶中杜仲总苷和杜仲黄酮的研究

Isolation of total glycosides and flavonoids from Eucommia ulmoides leaf by macroporous resin

摘要

摘要: 本文就9种大孔树脂吸附分离杜仲总苷和杜仲黄酮的工艺进行了研究，结果表明：一次性分离杜仲总苷(富含绿原酸、桃叶珊瑚苷等活性物质)和杜仲黄酮的适宜的大孔树脂为XDA-1和X-5。适宜的吸附解吸条件分别为：上柱液pH 6，吸附时间应大于10 h；XDA-1树脂用40%乙醇洗脱杜仲总苷，用80%的乙醇洗脱黄酮；X-5树脂用30%的乙醇洗脱杜仲总苷，用70%的乙醇洗脱黄酮。两种树脂的分离效果分别为：XDA-1分离杜仲总苷(其中绿原酸含量为14.23%，桃叶珊瑚苷含量为7.69%)粗品得率为8.01%，杜仲总黄酮粗品得率为4.76%，含量为15.82%；X-5分离杜仲总苷(其中绿原酸含量为15.39%，桃叶珊瑚苷含量为9.07%)粗品得率为7.35%，杜仲总黄酮粗品得率为5.11%，含量为16.15%。

Abstract: Technology for the isolation of total glycosides and flavonides from the leaves of Eucommia ulmoides by 9 kinds of macroporous resins was studied. Results show that the optimum resins are XDA-1 and X-5. The technical conditions for the separation are as follows: pH value of the sample solution loaded: 6, static absorption time: above 10 h, suitable desorption solvent to XDA-1: 40% ethanol for chlorogenic acid and aucubin, 80% ethanol for flavonoids; to X-5: 30% ethanol for chlorogenic acid and aucubin, and 70% ethanol for flavonids. The yield of total glycosides (crude product) extracted by using XDA-1 is 14.23%, in which the content of chlorogenic acid is 14.23%, of aucubin, 7.69%. The yield of flavonoids (crude product) extracted by using XDA-1 is 4.76% with the content of 15.82%. The yield of total glycosides (crude product) extracted by using X-5 is 7.35%, in which the content of chlorogenic acid is 15.39%, of aucubin, 9.07%. The yield of flavonoids (crude product) extracted by using X-5 is 5.11% with the content of 16.15%.

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