反相HPLC法快速测定鸭蛋蛋黄粉中胆固醇含量

基金项目: 教育部博士点基金(20020504008)

Quick determination of cholesterol content of dried duck egg yolk by RP-HPLC

摘要: 通过RP-HPLC方法，比较了不同的流动相、流速、检测波长和温度对鸭蛋蛋黄粉中胆固醇分离效果的影响。确定试验最佳条件为：流动相：乙睛：甲醇：异丙醇＝50∶80∶10(体积比)，柱温45℃，进样量20 μL，流速1.3 mL/min，检测波长205 nm。在此条件下测得的天然红心鸭蛋蛋黄粉和普通鸭蛋蛋黄粉中胆固醇的含量分别为24.84 mg/g和25.75 mg/g。
- 鸭蛋 /
- 胆固醇 /
- RP-HPLC
Abstract: Effects of different mobile phases, flow rates, dectecting warelengths and temperatures on cholesterol separation of dried duck egg yolk were studied by direct saponification-RPHPLC. The optimal chromatographic conditions of RP-HPLC are as follows: mobile phase is acetonitrile∶methol∶isopropanol=50∶80∶10(v/v/v); UV detecting wavelength is 205 nm; flow rate is 1.3 mL/min; column temperature is 45℃. Cholesterol content of the dried natural red yolk of duck egg and the common dried duck eggs yolk are 24.84 mg/g and 25.75 mg/g respectively under the above conditions.
- duck egg /
- cholesterol /
- RP-HPLC