Abstract: In this paper a fuzzy control and fuzzy decoupling method was proposed based on that temperature and humidity control of artificial climate chest with the characteristics of strong coupling and long time delay and their model parameters are uncertain and varying. This method need not object model and it can overcome object characteristics of strong coupling and long time delay. Furthermore system circuit of temperature and humidity control is given. The experimental result shows that this method has high precision and rapid responses. It can meet the demand of temperature and humidity control through artificial climate chest and achieve control precision of ±0.5℃ in practical application.