钙离子对盐胁迫下菊芋幼苗的生长、生理反应和光合能力的影响理论

    Effects of calcium ion on growth, physiological responses and photosynthetic ability in salt-stressed Jerusalem artichoke (Helianthus tuberosus L.) seedlings

    • 摘要: 以南芋2号为材料,研究了Ca2+对NaCl胁迫下菊芋叶片中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、净光合速率(Pn)和荧光参数及菊芋鲜重的影响,探讨了Ca2+在盐胁迫中的可能作用。结果表明,在1/2浓度的Hoagland营养液条件下外施10 mmol/L Ca2+对菊芋的生长没有明显的影响,但在150 mmol/L NaCl胁迫条件下,外施10 mmol/L Ca2+可有效防护胁迫所致的氧化损伤,从而维持较高的SOD活性,抑制脂质过氧化作用。使叶片在盐胁迫条件下,维持较高的PSⅡ的电子传递强度(Fm/Fo)、PSⅡ光化学效率(Fv/Fm)、PSⅡ量子效率(φPSⅡ)、光化学淬灭系数(qP)、Pn和较低的非光化学淬灭系数(qNP),有利于物质积累从而使生物产量增加。这主要归因于Ca2+可在一定程度上弥补盐胁迫导致Ca2+亏缺造成的离子失衡,使植物维持较正常的生理活动,稳定细胞膜结构,维持体内离子吸收平衡,保护光合机构。

       

      Abstract: Jerusalem artichoke (Nanyu 2) was used to study the effect of calcium ion on superoxide dismutase(SOD) activity, malondialdehyde(MDA) content, net photosynthetic rate, fluorescent parameters and fresh weight under NaCl stress. The results indicated that the addition of Ca2+ did not significantly influence the growth of Jerusalem artichoke under half-strength Hoagland nutrient solution treatment but markedly protected Jerusalem artichoke leaves from oxidative damage caused by 150 mmol/L NaCl stress. Therefore, higher activity of SOD and lower content of MDA in Jerusalem artichoke leaves were maintained under salt stress. As a result, the increases in status of electron transport by PSⅡ(Fm/Fo), primary photochemical efficiency of PSⅡ(Fv/Fm), quantum efficiency of photosystem Ⅱ(φPSⅡ), photochemical quenching coefficient(qP), net photosynthetic rate(Pn) and the decrease in non-photochemical quenching coefficient(qNP) were observed under salt stress. All these were favorable to the dry matter accumulation so that the fresh weight was increased significantly. In conclusion, the addition of Ca2+ compensated the deficient Ca2+ induced by salt stress, made the normal metabolism and the intact cell membrane, balanced the ions absorbed and protected the photosynthetic system.

       

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