Abstract: The feasibility of preparing microcapsule with dried yeast(S. cerevisiae) cell as wall materials was studied. The clove bud oil(CBO) was selected as core materials. The conditions for CBO microencapsulation were optimized by orthogonal experiment. Results show that the encapsulating yield of CBO reaches 41.26% under the conditions of encapsulating temperature of 70℃, encapsulating time of 9 h and the ratio of CBO mass to yeast mass of 1∶1. The morphology of the CBO microcapsule observed with optical microscope and scanning electron microscope(SEM) is global, and the particle diameter is 2.0～4.0 μm. The twinkling CBO drip can be found in the yeast cytoplasm. After being encapsulated in yeast cell, the volatile velocity of CBO is reduced by 43.25%. The antiseptic effect of CBO encapsulated by dried yeast cell is better than that of the equal quantity of CBO, and the microcapsule has some sustained-release antiseptic effect.