Abstract: The Emulation of rapeseed oil deodorizer distillate esterification by lipase reaction based on artificial neural network and central composite experiment was carried out. Back-Propagation algorithm with momentous factorwas adopted to train the neural network followed by the prediction of esterification reaction. Results show that lipase reaction is well simulated and the optimal technological conditions of lipase reaction can be obtained by the trained neural network. Under these conditions, the content of fatty acid methyl ester andthe recovery of vitamin E reaches 55.2% and 90%, respectively. It provids asignificant approach for the prediction of esterification of deodorizer distillate.