Abstract: To study the application of pokeberry pigment in the food containing Fe(Ⅲ), with fresh pokeberry juice as experimental material, the effects of Fe(Ⅲ) and EDTA-2Na in food on the stability of pokeberry pigment were investigatedby adding Fe(Ⅲ) and EDTA-2Na in the pigment solution. Results show that Fe(Ⅲ)can reduce the thermal stability and absorbance retention of the pigment; the stability of pigment has negative correlation with the density of Fe(Ⅲ) and heating time; adding EDTA-2Na in the food containing Fe(Ⅲ) can improve the stability of pokeberry pigment. When the Mole ratio of EDTA-2Na to Fe(Ⅲ) is more thanone, pigment absorbance(ABS) retention is above 85%; the stability of the pigment is related to the adding sequence of Fe(Ⅲ) and EDTA-2Na, and the pigment absorbance retention for "Fe(Ⅲ)+EDTA-2Na+pigment" is the best.