高压脉冲电场对植物乳杆菌的杀菌效果及三种模型的比较分析

    Inactivation effect of pulsed electric field on Lactobacillu plantarum in apple juice and comparative analyses of three models

    • 摘要: 研究了高压脉冲电场(pulsed electric field, PEF)对接种于苹果汁中植物乳杆菌(Lactobacillu plantarum)的杀菌效果并应用三种模型进行了分析。结果表明,随电场强度和脉冲时间增加,PEF对植物乳杆菌的杀灭效果增强,34 kV/cm、1050 μs时植物乳杆菌最大降低了4.135个数量级。Hülsheger 模型、Weibull模型和Log-Logistic模型均能很好地拟合PEF处理植物乳杆菌的失活曲线,五个模型评价参数,精确因子(Af),偏差因子(Bf),根平方和(SS),根平方方差(RMSE)和决定系数(R2)分析表明三个模型中,Log-Logistic模型最好地拟合了PEF处理下植物乳杆菌失活动力学变化。

       

      Abstract: The inactivation effect of pulsed electric field(PEF) on Lactobacillu plantarum inoculated into apple juice and model analysis were investigated. The inactivation level on Lactobacillu plantarum increased with the increment of the electric field strength and the treatment time. About 4.135 log maximum reduction of Lactobacillu plantarum was achieved at 34 kV/cm for 1050 μs. The survival rate curves were fitted by the Hülsheger, Weibull and Log-logistic models, and the fitting effects of the three models were evaluated by using a series of indices including Accuracy Factor(Af), Bias Factor(Bf), the Sum of the Squares of the differences of the natural logarithm of the observed and predicted data(SS), the correlation coefficient(R2) and the Root Mean Square Error(RMSE) between the observed data and the predicted data. The comparison of these indices indicates that Log-logistic model can provide best fitness for the inactivation by PEF.

       

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