Abstract: In rainy and humid regions, waterlogging stress with air temperature and humidity affects crop growth. Experimental study show that: a) there is a close correlation between relative yield of cotton and many factors such as waterlogging stress, air temperature and humidity when the groundwater table descending down from the land surface to a certain level. Among the influential factors the waterlogging stress ranked the first place; b) when cotton plants at a critical phase suffered one process of the waterlogging stress, the influence of subsurface waterlogging on the crop took the second place among the influential factors when the daily temperature was normal, however, in case of daily maximum temperature no less than 35℃ within ten days after surface waterlogging, the influence of subsurface waterlogging on the crop was less than that of high temperature weather on it; c) in the case of multiple waterlogged process taking place one by one, the influence upon the crop yield from the continuous days that daily maximum temperature was no less than 35℃ within ten days after surface waterlogging was greater than that from the continuous days of groundwater depth less than 80 cm.