Abstract: Experiments were conducted to observe the effects of polypropylene net ball, fiberglass and carbon fiber film carriers on biogas production, pH values, COD removal rate, microorganism and activity of coenzyme F₄₂₀ in the outflow fermented and fermentation rules in the anaerobic condition of 5% cow manure. pH values decreased to the lowest at the third day of fermentation, then increased above 7 at the 7th day of it. Biogas output reached the highest pinnacle at the ninth day of it, then decreased gradually. COD removing happened mainly during 4～8 day of fermentation. The amount of bacteria in the outflow fermented was the highest at the sixth day of it, then decreased to relatively stabilizing level during 12～26 day of it. The activity of coenzyme F₄₂₀ was the same changing rule as the amount of bacteria in the outflow fermented. Polypropylene adhered more anaerobic microbes to its surface, and prolonged pinnacle significantly and increased biogas yield(p<0.05) and reduced significantly outflow of microorganism with outflow of fermented manure(p<0.01).