Abstract: Taking the sunflower meal of aqueous enzymatic extraction oil as material, sunflower isolated proteins were prepared and analyzed for proximate composition and amino acid content, and their functional properties were also investigated. Results show that sunflower protein isolates contain less lysine than the FAO pattern of essential amino acid requirements, the functional properties of sunflower protein isolates are better than or close to that of soybean isolated proteins. The proteins were characterized by gel filtration and gel electrophoresis. Isolated proteins show three major fractions, which molecular weights are 380×10³, 100×10³ and 27×10³, respectively. Chlorogenic acid is shown to be more associated with isolated proteins.