叔丁醇体系脂肪酶催化菜籽油制备生物柴油

Preparation of bio-diesel from rapeseed oil catalyzed by lipase in t-BuOH

摘要: 为了在生物柴油制备中改善酶的催化环境，该文通过对脂肪酶LVK催化菜籽油乙醇解反应几个主要影响因素的研究，得出其最佳反应条件为：醇油摩尔比5∶1，催化剂用量10%，叔丁醇用量10%，反应温度40℃，反应时间为30 h，转酯率达到94.7%。研究表明10%的叔丁醇体系能完全解除乙醇对脂肪酶LVK的催化抑制作用；还消解除副产物甘油对脂肪酶LVK的失活。

Abstract: To improve catalytical environment of lipase, factors influencing rapeseed oil ethanolysis reaction catalyzed by lipase LVK were investigated. And the optimal reaction conditions were attained as follows: 5∶1 of ethanol/oil molar ratio, 10% enzyme and 10% t-BuOH based on oil weight, reaction at 40℃ for 30 h, under which the maximum ethyl esters yield can reach 94.7%. The experimental results showed that t-BuOH(10%) could relieve the inhibition of ethanol on lipase LVK completely in the reaction system, and relieve deactivation of by-product glycerol on lipase LVK.