Abstract: To explore plant extracts as antifungal agents for the postharvest diseases control of banana, the antifungal activities against Colletotrichum musae in vitro of the methanol extract, its ethyl acetate fraction and n-butanol fraction of the rhizomes of Tupistra chinensis were evaluated by spore germination and mycelial growth rate. The stability of antifungal activity of the n-butanol fraction of the methanol extract resisting high temperature(121℃ for 20 min), UV irradiation(30 W for 15 h, 50 cm vertical distance) and acid (pH=3) was investigated. Furthermore, the storage experiments of postharvest banana fruits dipped in the solutions of methanol extract and its two fractions were carried out separately. Results show that the EC₅₀ values of methanol extract, its ethyl acetate fraction and n-butanol fraction inhibiting the spore germination of Colletotrichum musae are 1490.27, 638.40, 1112.65 μg/mL, and the EC₅₀ values inhibiting the mycelial growth of Colletotrichum musae are 1262.16, 451.02, 955.58 μg/mL, respectively. Treatment of high temperature, strong acid and UV irradiation do not affect the antifungal activity of the n-butanol fraction of the methanol extract of Tupistra chinensis against Colletotrichum musae. Application of the methanol extract, its ethyl acetate fraction and n-butanol fraction lead to a significant decrease in the incidence of latent anthracnose during storage of postharvest banana fruits.