Abstract: De-coloration experiments of burdock oligosaccharides by exchange resins and absorption resins were studied. The effects of six resins on the de-coloration rate of burdock oligosaccharides were investigated. Results show that absorption resins DA201-B and DA201-C, anion exchange resin D301-G represent good de-colorizing abilities. De-colorizing experiments were carried out through orthogonal experiment. The optimum technological parameters are as follows: D301-G resin at 35℃ with pH 6.5. The de-coloration rate is 95%, the polysaccharides holding rate is 75%, the protein removal rate is 87%. The results also indicate that burdock colorants are most possibly small-molecular-weight substances with negative charge.