基于酶抑法的农产品农药残留快速检测系统及影响因素分析
Rapid detection system development and influence factor analysis for pesticide residues in agricultural products by enzyme inhibition method
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摘要: 为快速检测农产品农药残留，开发了基于酶抑制法的农产品农药残留快速检测系统。该检测系统由固体光源、样品池、单片机测量电路板及微机等组成，通过测量经酶抑制反应后样品液的吸光度值实现农产品中农药残留的快速检测。研究了吸光度随反应时间的变化关系、温度对酶活性的影响及农药残留浓度与酶抑制率的关系。结果表明吸光度随反应时间逐渐增加，反应前期增速快，线性度好，适合测量；酶在20～40℃时具有最佳的活性，灵敏度高；农药残留对酶的抑制作用明显，当抑制率高于70%时可判定农药残留超标。Abstract: In order to detect the pesticide residues quickly in farm products, a rapid detection system was developed to determine the pesticide residues in agricultural products based on the inhibition of organophosphates against acrtyl-cholinesterase. The system mainly consists of a solid light, a sample container to store the liquid, a micro-controller based testing circuit and a personal computer. The amount of pesticide residues was determined by measuring the light absorbency of the sample liquid after inhibition reaction. The changes of light absorbency with reacting time, the influence of temperature on the activity of enzyme, and the relationship between the concentration of pesticide residues and the inhibition rate of enzyme were investigated. The results show that the light absorbency increases with reacting time, and the increment is much higher in initial reacting period; the enzyme has good performance at the temperature of 20～40℃; the inhibition rate of pesticide residues on the activity of enzyme is distinct, and it can be determined that the pesticide residues exceed the normal level when the inhibition rate exceeds 70 percent.
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