Abstract: A new method for sterilizing the cutting tools of fruit tree aseptic culture during the fruit tree tissue culture and propagation in vitro was introduced. A newly roboticized heater with modern control and heating technology was used in this method. An innocuous, flavourless and high-temperature-enduring solid material was selected and processed to be the 2 mm-diameter spheroid as a specific sterilization medium. After heating the medium, the heat storing endurance was used to sterilize contactly the cutting tools of aseptic operation, and good results were continuously obtained for three years. By this method, the disadvantages of low speed, high energy consumption, serious air pollution and possibility to set off fire of traditional alcohol burner sterilization were overcome. As a result, the speed of fruit tree propagation in vitro was increased by 15.38%, the energy consumption was decreased greatly, and the air pollution and possibility to set off fire were avoided thoroughly as well.