Abstract: Xanthoceras sorbifolia bunge seeds were taken as raw materials and the ultrasonic-assisted extraction technology was used to extract seed oil. The single factor experiment and quadratic orthogonal rotation regression combination design were adopted to study the effects of such factors as solid-liquid ratio, ultrasonic frequency and time on oil extraction rate. The optimal technological condition of oil extraction from Xanthoceras sorbifolia bunge seeds by ultrasonic was determined, then the extraction regression mathematical model was established. The order of factors that influence the oil extraction rate within the experimental range was as follows: extraction time>solid-liquid ratio>extraction temperature>ultrasonic frequency. The optimal technological parameters of oil extraction from Xanthoceras sorbifolia bunge seeds using petroleum ether with boiling range of 60～90℃ as the solvent should be as follows: solid-liquid ratio 1∶10(g/mL), extraction temperature 60℃, extraction time 35 min, ultrasonic frequency 60 kHz, and under such conditions, the total oil extraction rate after extraction three times is 60.18％, extraction ratio can reach as high as 92.47%, and the established regression mathematical model can predict the oil extraction rate of Xanthoceras sorbifolia bunge seeds accurately.