4株红螺菌科细菌的拮抗作用及其影响因素
Antagonistic effects of four strains of Rhodospirillaceae and factors affecting inhibitory function
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摘要: 对4株红螺菌（荚膜红假单胞菌PSB-2、球形红假单胞菌PSB-3、胶质红假单胞菌H1和沼泽红假单胞菌H10）的去细胞上清液（CFS）抑制水产养殖病原菌的作用进行研究，并探讨养殖水体环境对红螺菌拮抗作用的影响。结果表明，4株红螺菌在细胞生长稳定期后能有效分泌拮抗物质，其去细胞上清液（CFS）对20多株水产养殖病原菌均有抑制作用。从抑菌活性看，荚膜红假单胞菌的抑菌活性强于其他菌株：荚膜红假单胞菌对水产养殖病原菌的最低抑菌浓度（MIC）为8～64 AU/mL，而其他红螺菌株多为4～16 AU/mL。在养殖水体的生态因子中，水体pH、NH4+-N与NO2?-N对红螺菌代谢产物的抑菌活性影响较大，pH7～9，NH4+-N低于5 mg/L、NO2?-N浓度低于7mg/L时抑菌活性较强，而养殖水体温度与溶氧变化对红螺菌的抑菌活性基本无影响作用。Abstract: The inhibitory effects of cell-free supernatant (CFS）produced by four strains of Rhodospirillaceae (Rps.capsulata PSB-2, Rps.sphaeroides PSB-3, Rps.gelatinosa H1 and Rps.palustris H10） against pathogenic bacterium were assayed. And the influences of aquaculture environmental factors on antagonistic effects of metabolic products were studied. The results show that antagonistic materials of four strains of Rhodospillaceae were secreted after the stationary phase, and cell-free supernatant (CFS） had inhibitory ability against more than twenty pathogenic bacteria. As for inhibitory activity， Rps.capsulata had the strongest antibacterial activity among four strains of Rhodospirillaceae, and its MIC against pathogenic bacteria was 8～64AU/mL. MIC of other three strains of Rhodospirillaceae were 4～16AU/mL. Antibacterial activities of Rhodospirillaceae were affected significantly by pH value and the concentrations of NH4+-N and NO2?-N: antimicrobial effects were greatly significant between pH 7 and pH 9, and under the concentrations of NH4+-N below 5 mg/L and NO2?-N below 7 mg/L, but antimicrobial activities were not sensitive to the changes of temperature and Dissolved Oxygen (DO) in aquaculture water.
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Keywords:
- Rhodospirillaceae /
- pathogenic bacteria /
- antagonistic effect /
- environmental factor
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