Abstract: On the basis of theory of data envelopment analysis, mathematical model and model signification for evaluating the influences of electric field on the biological effect were given. The method provides a new tool for researching the relationship between the biological effect and different electric field strengths. By means of these models the comprehensive information on the influences of electric field on the seedling length, root length of the seedling, wet weight, dry weight, POD activity, SOD activity and MDA contain of wheat seeds was provided. The results show that the different electric field strength has a different influence on seedling growth and biological process, and the indexes of biological effect vibrate with the increase of the electric field from 0.5 kV/cm to 6.0 kV/cm. The best condition of electric field is 2.5 kV/cm and 4.0 kV/cm.