Abstract: Based on TM images in 1999, ETM+ images in 2002, ASTER images in 2004 and basic background data in the main stream of Tarim river region, technically supported by the data warehouse, the theory of spatial data and attribute data integration and the seamless integration of multi-source data, a spatial database is created to store the data about basic background data, remote sensing images, thematic data, and attribute data. By using the technologies of RS, GIS and GPS etc., land use information is obtained and analyzed in the main stream of Tarim river in 1999, 2002 and 2004. The research exposes the changing range, annual variation of land use, and the spatio-temporal changing trend of the farmland in researched area after emergent water transportation. The results show that the utilized areas of farmland, natural grassland, reed land and bottomland increased quickly in the main stream of Tarim river from 1999 to 2004, while the utilized areas of woodland, building land, water area, waste grassland, and saline land decreased correspondingly. The farmland increase at the cost of vegetation destroying and water area decreasing, which aggravates the conflict between human activity and nature environment, and deterioration of environment.