Abstract: In order to study the effects of land use change on ecosystem service values, change of land use in Yuanmou County from 1986 to 2000 was analyzed by use of the LANDSAT TM images and remote sensing and geographic information system. Ecosystem service coefficients of different land use types in the study area was estimated based on them found by Xie Gaodi and the ecosystem services sensitivity analysis was conducted to determine whether these coefficients can be used in the study area or not. The results show that the estimated area of cultivated land, pasture land, water area and unused land decreased but that of forest land and construction land increased from 1986 to 2005. The total ecosystem services value in the study area in 2000 is higher of 3989736.42 Yuan than that in 1986 for the increase of forest land area and its highest ecosystem services coefficients. It can be concluded that ecosystem service value is determined by land use type. The coefficient sensitivity(CS) of the study indicates that it is reasonable for the coefficient to be used in the study area.