Abstract: From the view of the three multilevel spatial scales in Fujian region (including province, city, county) and application feature of agricultural spatial information, the multi-resource and multi-scale agricultural spatial information sharing and web service were studied based on network. By integration of spatial information system and Web services technology, the key technology of three multilevel spatial scale system of agriculture spatial information sharing and service in Fujian Province (Fujian province on 1︰250000 scale, and Zhangzhou city on 1︰50000 sacle, and Longhai county and Zhangpu county on 1︰10000 scale) was investigated, including data standardization and integration, operation model of agricultural application, information exchanging and sharing system structure and metadata catalog services, and so on. The agriculture basic database groups were built by standardizing the conformity of multi-source and multi-scale agriculture data. And the newly designed conformity operation model which has thrashed out a series of key technology of information process and service problems can meet the requirements of different application targets and service contents. Based on XML Web services technology and information system, the agriculture geo-information service platform of multi-scale application in Fujian Province was implemented, which promoted the spatial information application and service on the regional agriculture.