石榴果皮中抑菌活性物质提取工艺优化(简报)

Optimization of technology for extracting antibacterial substances from pomegranate peel

摘要: 为了研究石榴果皮中抑菌活性物质的提取工艺，以金黄色葡萄球菌做指示菌，以抑菌圈直径为活性追踪指标，在单因素试验的基础上通过二次回归正交旋转组合设计进行工艺参数优化。结果表明，对石榴果皮抑菌活性物质提取效果的影响大小顺序为：乙醇浓度、提取温度、液固比、提取时间；其最佳提取工艺条件为乙醇浓度60%，液固比10︰1，提取温度60℃，提取时间4 h。在此优化条件下，石榴果皮提取液抑菌圈直径为16.30 mm。

Abstract: In order to investigate the technology for extracting antibacterial substances from pomegranate peel, Staphylococcus aureus was used as indicator bacteria and diameter of inhibition zone was used as the activity monitoring parameter. The optimum extraction conditions were obtained by quadric regression orthogonal rotational experiments. The results show that the order of the main factors influencing the extracting effect from more important to less are as follows: ethanol concentration, extraction temperature, ratio of liquid to solid and extraction time; the optimum extraction conditions are that the concentration of ethanol is 60%, the ratio of liquid to solid is 10︰1, the temperature of extraction is 60℃ and the time of extraction is 4 h. Under the above mentioned conditions, the diameter of inhibition zone is 16.30 mm.