Abstract: This article intends to offer scientific basis for samplings arrangement, information sources selection, optimum remote sensing temporal selection, crop discrimination and cotton area estimation. Based on the regionalization principle, basis and index of the cotton monitoring using remote sensing, according to the database of remote sensing images of Xinjiang, the geography foundation and the special subject images database, the Stat. databases, combining the spectrum measurements of crops and crop phenological calendar, the analytical research was carried out. The results indicate that: the three main cotton regions in Xinjiang region could use land resources satellite as information sources; in all the cotton regions, with the scheme for the regionalization of the cotton remote sensing information sources, the optimum remote sensing temporal for the three cotton regions is September, the second temporal is June, but in TULUPAN, it is late August and the early and middle June; based on the results, the three cotton regions in Xinjiang region could be divided into six subareas, which are main cotton regions, heavy proportion cotton region, inferior heavy cotton region, inferior cotton region, sparse cotton region and no cotton region.