冷却肉中假单胞菌温度预测模型的建立与验证

    Development of models to predict the growth of Pseudomonas isolated from chilling pork

    • 摘要: 为了快速预测和监控引起冷却肉腐败变质的主要微生物——假单胞菌的生长,以从冷却猪肉中分离得到的假单胞菌P-1菌株作为受试菌,建立和验证0~10℃低温条件下假单胞菌的生长动力学模型。利用SAS程序拟合不同温度条件下的生长情况,经过比较发现,Gompertz模型比线性模型能更好地拟合假单胞菌的生长,从而得到假单胞菌生长的Gompertz模型参数;利用平方根模型对假单胞菌的最大比生长速率平方根-温度进行拟合,得到假单胞菌生长的二级模型;利用培养基数据、冷却肉产品数据和温度波动条件下的数据对所得到的二级模型进行验证,计算得到总的偏差因子和准确因子分别为0.944和1.256,结果表明建立的二级模型能真实快速有效地预测0~10℃下冷却肉中假单胞菌的生长。

       

      Abstract: To quickly predict and monitor the growth of Pseudomonas P-1 causing decay of pork at 0~10℃ isolated from chilling pork, the growth model was developed. The growth condition of Pseudomonas P-1 was simulated at different temperatures using the SAS program. Compared results show that the estimated parameters from Gompertz equation for the assayed results show better predictive ability than that of linear model. Square-root model was applied to simulate and integrate the secondary model of Pseudomonas. The validation of the secondary model was based on culture data, chilling pork data and fluctuate temperature data. The average bias factor and accuracy factor are 0.944 and 1.256 respectively. The research results indicate that the secondary model is valuable for rapid and precise prediction of the growth of Pseudomonas at 0~10℃.

       

    /

    返回文章
    返回