Abstract: The rheological behaviors and viscoelastic properties of (1→3), (1→4)- β-D-Glucan isolated from oats of China were investigated .The rheological properties and effect of concentration, molecular mass and temperature on viscoelastic of β-Glucan were studied using AR-500 rheometers. The results show that oat β-Glucan solution exhibited a shear-thinning behavior. When the concentration of β-Glucan increased from 0.1% to 1%, the flow index of power law equation come from flow curves was reduced from 0.998 to 0.842. The viscosity of oat β-Glucan solution was in direct ratio with molecular mass of β-Glucan and in inverse ratio with solution temperature. Increased the molecular mass of β-Glucan reduced the flow index of power law equation. The viscosity of oat β-Glucan solution decreased in acidity or alkalescence condition. The viscoelastic properties of β-Glucan was influenced by concentration, molecular mass of β-Glucan and solution temperature. The elastic modulus of liquid was enhanced and viscous modulus of liquid was reduced when the concentration or molecular mass of β-Glucan increased. Both elastic modulus and viscous modulus of liquid were reduced when the liquid temperature increased.