Abstract: Different factors that influence hydrolysis rate of corn stover by dilute sulfuric acid hydrolyzing method were studied, including hydrolyzing time, temperature, concentration of sulfuric acid and solid content. The experimental results show that the majority hydrolyzed part of corn stover was hemi-cellulose. The hydrolysis of cellulose needs a longer time, higher temperature and higher sulfuric acid concentration. The influences of temperature, hydrolyzing time, concentration of the dilute sulfuric acid and the solid content in hydrolyzing process were very prominent, especially the temperature and the concentration of the dilute sulfuric acid. The hydrolysis goes on efficiently when the temperature was higher than 100℃, and the cellulose began to hydrolyze when it was higher than 110℃. The hydrolysis of corn stover was completed in 40 minutes; more hydrolyzing time cannot increase the hydrolysis rate. The proper concentration of sulfuric acid is 1.5%, higher concentration also cannot increase the hydrolysis rate. The solid content also influence the hydrolyzing process, lower solid content can acquire higher hydrolysis rate. From the experiment we also find the best treatment at following condition: 120℃, 80 min, 1.5 percent of diluted sulfuric acid, 10 percent of solid content in hydrolyzing process, and under this condition 32.71% reductive sugar in mass can be obtained compared with corn stover.