芽孢杆菌组合CL-8发酵条件优化及其防病效能研究（简报）

吴 艳; 陶 晶; 赵思峰; 闫豫君; 李 晖; 李 春

芽孢杆菌组合CL-8发酵条件优化及其防病效能研究（简报）

Optimizing the fermentation conditions for Bacillus sp. combination CL-8 thalli with cooperative and synergistic action and its biocontrol efficacy

摘要

摘要: 为了使生防菌Bacillus sp. 组合CL-8发酵水平达到最佳效果，为其工业化发酵生产和田间应用提供参考，通过单因素实验和正交实验，确定了Bacillus sp. 组合CL-8最佳发酵培养基和最佳发酵条件。最佳发酵培养基为：玉米粉10 g/L、豆粕10 g/L、蜂蜜 5 g/L、氯化钠 5 g/L、碳酸钠 2 g/L、磷酸氢二钾 2 g/L、硫酸镁 0.2 g/L、碳酸钙 1 g/L、硫酸锰 0.1 g/L、氯化铁 0.1 g/L；最佳发酵条件为：三个单一拮抗菌株Bacillus sp. SL-13、SL-14和SL-44最佳接种比例为 2︰1︰2，培养基初始pH值为7.0。组合CL-8发酵液对加工番茄种子浸种后可提高加工番茄的防病效果，达到55.53%，明显高于三株单一菌浸种后的效果。

Abstract: The fermentation conditions for antagonistic combination Bacillus sp. CL-8 thalli were studied through single factor and orthogonal experiment. It was demonstrated that the optimum fermentation media contained corn meal 1%, bean pulp 1%, honey 0.5%, NaCl 0.5%，Na2CO3 0.2%, K2HPO4 0.2%, MgSO4?7H2O 0.02%, CaCO3 0.1%, MnSO4?H2O 0.01% and FeCl3 0.01%. The favorable fermentation conditions are as follows: the proportions of Bacillus sp. SL-13, Bacillus sp. SL-14 and Bacillus sp. SL-44 are 2︰1︰2 and initial pH value of the medium is 7.0. Bacillus sp. combination CL-8 thalli cultured under the optimum conditions was also used to inhibit Rhizoctonia solani and control damping-off of processing tomato seedling in the field. The biocontrol efficacy was 55.53%，which was much better than that by three single Bacillus sp. SL-13, SL-14 and SL-44.

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