Abstract: Due to lutein loss in traditional production, a biological procedure described for Tagetes erecta in this article combines silage with enzymatic hydrolysis of the plant cell wall. To take lutein extraction rate as an index, the ensilage process parameters were determined, and enzyme activity in the water-organic solvent coexistence system had been analyzed. The results indicated that lutein has a loss in both ensilage and drying process, and lutein in the drying process (16.33%) compared to ensilage process (6.55%, 10.27%) loses more. Adding β-glucosan enzyme or liquid cellulose in the ensilage stage at 30℃ for 5 d may well degenerate plant cell wall, which is useful for lutein extraction. To take Tagetes erecta frozen dried powder, ensilaged fresh flower as raw materials, the suitable water yield 6.8, 2.8～3.8 mL/g respectively that the extraction process needed in the water-organic solvent coexistence system was determined, which has guaranteed a good enzyme activity, it can degrade the plant cell wall to make hydrolysis carry on smoothly. Through the check experiment we have also found cellulase a higher activity in the enzyme-water-organic solvent coexistence system.