Abstract: To improve the cleaning performance of micro-combine harvester of wheat, facilitate applying of production, and meet requirements of cleaning performance of the micro-combine wheat harvesters, the optimum combination between structural parameters and motion parameters on cyclone separating system of no-oriented films are tested and verified based on orthogonal test and general rotary unitized test. The experimental results are analyzed by variance analysis method and regression analysis method. The results show that the rotate speed of catching impurity fan, the rotate speed of winnower, and the top cone angle have more evident effects on the cleaning rate and the loss rate of seeds than the height of separating barrel. The results can provide design reference for cleaning system of micro-combine harvester of wheat. When the rotate speed of catching impurity fan is 2399 r/min and the rotate speed of winnower is 1097 r/min, the cleaning rate is 99.15 percent and the loss rate is 0.295 percent.