利用甜高粱秸秆汁发酵生产丁醇、丙酮

Production of acetone and butanol by fermentation of sweet sorghum stalk juice

摘要: 该试验以甜高粱秸秆汁作为生产丙酮、丁醇的发酵原料，从5种丙酮-丁醇菌中选出能够利用甜高粱秸秆汁且丁醇产量高的Bacillus acetobutylicum Bd3菌作为试验菌株，并对该菌株的发酵条件进行优化，得到的优化条件为：糖度为10oBrix的甜高粱秸秆汁，玉米浆含量5 g/L，接种量6%(v/v)，(NH4)2SO4 5 g/L，KH2PO4 0. 4 g/L，CaCO3 6 g/L，温度32℃，pH6.8，丁醇产量达到10.29 g/L。

Abstract: Sweet sorghum stalk juice was used as raw material for producing acetone and butanol by fermentation. Bacillus acetobutylicum Bd3 was selected from five different acetone-butanol producing strains, as experimental bacterium which produced more butanol than the four others. And the conditions of acetone-butanol fermentation by Bd3 were optimized: sweet sorghum stalk juice10oBrix, corn-steep 5 g/L, inoculation 6%(V/V), (NH4)2SO4 5 g/L, KH2PO4 0.4 g/L, CaCO3 6 g/L, temperture 32℃, pH6.8, the output of butanol achieved 10.29 g/L.