Abstract: In order to develop an environmental friendly bamboo particleboard with bio-based adhesive, effects of coating ratio of soy protein adhesive modified by sodium dodecyl sulfate on the physical and mechanical properties of bamboo particle board were studied. The physical and mechanical properties of bamboo particleboard based on sodium dodecyl sulfate modified soy protein adhesive as affected by coating ratio were studied. The results showed that the optimal coating ratio of SPI adhesive was 13%. The mechanical properties and water resistance of particleboards affected by moisture content, press time, press temperature were tested by Response Surface Methodology. Optimal properties of particleboard were obtained with moisture content 26%, press temperature 175℃ and press time 8.5 min. Under the optimal conditions, all the physical and mechanical properties of particleboard achieved the requirements of GB 4897－2003, with internal bonding 0.65 MPa, modulus of rupture 16.8 MPa , thickness swelling rate 6.7%.