Abstract: Three kinds of fresh water fish, such as Lateolabrax japonicus, Aristichthys nobilis and Puntius lineatus, and three kinds of marine fish, such as Pampusargenteus, Pseudosciaenapolyactis and Silurus meridionalis, were analyzed for the evaluation of quality and freshness by the electronic tongue, which was developed by author’s research group. Fresh water fishes and marine fishes were all stored at 4℃. Five pieces of each fish were cut for analysis by electronic tongue each day. The results showed that the quality property and freshness of the fish at different time intervals could be efficiently separated by the electronic tongue. The electronic tongue could discriminate not only between fresh water fishes and marine fishes, but also different types of fresh water fish or marine fish. The electronic tongue as a kind of modern intelligent sensory instrument shows a great potential in the evaluation of quality and freshness for fish.