Abstract: In order to improve the slow-release performance of Matrix-Based Fertilizer, ten kinds of Matrix-Based Fertilizer were made with organic and inorganic substrates. Their nitrogen releasing orders were studied by alternate leaching method in sandy pole, and the slow-release effects of ten substrates were evaluated through the parameters of Logistic equations and Unweighted Pair-Group Method Using Arithmetic Averages cluster analysis of nitrogen releasing inflection. The result showed that the dynamics of nitrogen accumulated releasing rate could be quantitatively described by four equations: the first-order Kinetics equation, Elovich equation, Parabola equation and Logistic equation. However, the imitation of Logistic equation was the best. That was to say the Logistic equation was the most practical for imitating nitrogen release mechanism of MBF. Oxidized starch and lignin were the best slow-release organic substrate and bentonite was the best inorganic one.