Abstract: The acoustic frequency technology is to treat the plant with a specific frequency sound wave. Acoustic studies have found that plants can produce low frequency sound spontaneously. With the addition of the technology, the specific frequency sounds to make a match-absorption and resonance on the target plants. Thus, the technology strengthens photosynthesis and speeds of cell split and makes plant grow and develop faster. Treated plants are blooming and bearing fruits ahead of time. Acoustic frequency technology was applied to sweet pepper, cucumber and tomato in greenhouse. Various controlled experiments were made and all results indicted that the technology could increase the output of vegetables notably, improve crops quality, strengthen the capability of disease-resistance. The yields of treated sweet pepper, cucumber and tomato were 63.05%, 67.1% and 13.2%, respectively higher than that of control group. Moreover, the incidence of treated tomato disease decreased by 6, 8, 9, 11 and 8 percentage points, respectively, including red spider, aphids, grey mold, late blight and virus disease.