Abstract: Cold storage combined with ozone treatment is a new method used in storage of fruits and vegetables. It is important to determine the concentrations of ozone produced by ozone generators with different capacities applied in different types of cold storage rooms for fruits and vegetables. The concentrations of ozone produced by three kinds of ozone generators (ozone yield: 0.2, 0.49 and 0.94 g/h, respectively) applied in two different types of cold storage rooms (volume of 100 m3 and temperature of (0±0.5)℃) were investigated. The results showed that the capacity of ozone generator had a significant influence on the ozone concentrations, which became higher as the capacity of ozone generator increased. The ozone concentration reached the maximum in Controlled Atmosphere(CA) storage room at 120～140 min and in brick masonry cold storage room at 60～90 min, then the ozone concentrations kept constant at the level of maximum concentration. In the CA storage room, the maximum ozone concentrations produced by the ozone generators (ozone yield: 0.94, 0.49 and 0.2 g/h, respectively) were 1.37, 0.71 and 0.30 mg/m3, respectively, and the correlation coefficients of regression curves between ozone concentrations and time were 0.9234, 0.9252 and 0.9689, respectively. While in the brick masonry cold storage room, the maximum ozone concentrations were 1.25, 0.62 and 0.23 mg/m3, respectively, and the correlation coefficients of regression curves were 0.7293, 0.8816 and 0.9105, respectively. The concentration of ozone accumulated in CA storage room was remarkably higher than that in brick masonry cold storage room.