蔬菜品质相关酶高温瞬时失活动力学

    Inactivity kinetics for quality-related enzymes in vegetable with high-temperature short-time treatment

    • 摘要: 为了对蔬菜的超高温杀菌过程进行有效的控制,利用“胶囊式”时间-温度积分器(TTIs,time-temperature integrator)对蔬菜中品质相关酶——过氧化物酶(POD)、多酚氧化酶(PPO)进行了高温(100~140℃)、瞬时(10~150 s)失活动力学研究,并运用数学模型测算了温度对该TTIs升温时间的影响。 研究结果显示,TTIs的最长升温时间不超过0.5 s,相对于热处理时间基本可以忽略;马铃薯中的POD和PPO为4种常见蔬菜中最耐热的酶,测定得到动力学参数,POD:Z= 66.76 ℃,D121.1=61.88 s,Ea =66.348 kJ/mol;PPO:Z= 37.55℃,D121.1=152.65 s,Ea =63.800 kJ/mol。该试验为超高温瞬时杀菌过程控制提供了关键参数,也为国内此领域研究补充了部分参考数据。

       

      Abstract: To execute effectively control to the ultra high temperature (UHT) sterilization for vegetables, inactivity kinetics for quality-related enzymes such as peroxidase (POD) and polyphenoloxidase (PPO) in common vegetables were studied by time-temperature integrator method (TTIs) under high temperature-short time treatments (100-140℃, 10-150 s). Effect of the temperature on the come-up time for TTIs was accurately calculated by mathematical model. The results showed that the coming-up time of TTIs was less than 0.5 s. Compared with heat treatment time,it could be ignored. Kinetic parameters for quality evaluation were Z=66.76℃, D121.1=61.88 s, Ea =66.348 kJ/mol for POD, and Z=37.55℃, D121.1=152.65 s, Ea =63.800 kJ/mol for PPO. The study provides key parameters for UHT sterilization control, and it makes up some data gaps in the domestic research field.

       

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