Abstract: In order to study the theory of ozone preservation, according to the relationship between the respiration of fruits and vegetables and the permeability of film, the respiration model was established in pack which used ozone as the fresh atmosphere. Package bag with composite film PA/PE containing lotus root slices was taken as example, and the model parameters were identified by genetic algorithm (GA). In addition, the changes of gaseous concentration in package bag at different temperatures were simulated. The results show that the simulative value and actual value can be well matched, thus the simulative value can provide academic guidance for the design of fresh-keeping and package for fruits and vegetables based on ozone.