Abstract: The technology of producing protein feed from distributional degradation of corn stalk by multi-strain was studied. Most of lignin was degraded through solid fermentation by Phanerochaete chrysosporium. Then Trichoderma viride was vaccinatated for further degradation of lignocellulose. Protein feed was produced by adding Candida utilis. The results manifested that the optimal technical conditions were as follows: Trichoderma viride inoculation time was the10th day; fermention time was 12 days; After the bran was added into stalk by the ratio of 1︰3.0, the best technical conditions were yeast inoculation 8%; mixture ratio of solid to liquid 1︰3.0; addition volume of ammonium sulfate 2.5%, continuous fermentation time 72 h. Under the optimal conditions, the content of crude protein was up to 25.3%. The results show that distributional degradation technology of producing protein feed by multi-strain provides a new method for utilization of corn stalk.