Abstract: For promoting the application and sharing of tobacco germplasm resources in China, a web-based Chinese tobacco germplasm resources information system (CTGRIS) was developed by using B/S (browser/server) architecture and ASP.NET technology. Microsoft SQL Server was used as database management system. Server-side assemblies were programmed by C#, and client-side (web page) was implemented by using XHTML with JavaScript. Based on two backend databases (characteristic database and common database), this system can provide five kinds of information services, including germplasm resources query, core collection query, tobacco image query, common data query and germplasm resources sharing. The CTGRIS is a powerful tool for tobacco scientific research, which will facilitate the information and germplasm resources sharing and exchange.