Abstract: In order to evaluate the effect of temperature, ammonia concentration and aeration time on nitrification characteristics of swine wastewater, Sequencing Batch Reactor Activated Sludge Process (SBR) was adopted to treat the swine wastewater with high ammonia nitrogen concentration in the non-limiting dissolved oxygen conditions. The results demonstrated that SBR technology could effectively remove ammonia nitrogen in swine wastewater, shortcut nitrification occurred during the whole experimental process. When the ammonia nitrogen concentration in the influence was less than 250 mg/L, the ammonia concentration and temperature (even when the temperature was at 15℃) had little effect on ammonia nitrogen removal and nitrite accumulation rate (both were about 80%). Prolonged aeration did not affect shortcut nitrification; nitrite accumulation resulted from co-inhibition of free ammonia (FA) and free nitrite (FNA) during biological treatment for high concentration of ammonia-nitrogen wastewater.