Abstract: The effects of post-harvest treatment with biological preservative C06 at two concentrations (50% and 100%) on the physiology and fresh-keeping of honey peaches (“Xinfengmilu”of Shanghai Nanhui District) were investigated at 3℃ storage. The results showed that the C06 treatment could dramatically affect the physiological changes after treatment. It decreased the loss of fruit firmness, inhabited membrane permeability and MDA level. The activities of POD, CAT and PPO showed a rise first and then a drop during storage and C06 treatment could significantly enhance anti-reversibility enzyme activity of CAT and POD, and inhibit PPO activity. After 21 days the percentage of healthy – fruit of 100% C06 treatment was 85%. The results demonstrate that the higher concentration C06 treatment is helpful for prolonging the storage life of peach at low temperature. Combined cost-effectiveness with practical production, 100% C06 treatment should be taken to honey peach preservation.