Abstract: Aiming to develop an industrial method for disrupting pollen, steam-explosion technique was studied. The optimal procedure was composed of the following steps. First, dry pollen was mixed with 0.05 mol/L of citrus acid solution to 40% concentration and homogenized by a constitution agitator. Then, the pollen paste was put into the material chamber, and heated to 0.15 MPa with 0.4 MPa supersaturated steam for 11 s. After the steam valve was closed and the material discharge valve was opened, the pollen paste was sprayed out of the material chamber, and pollen cells were exposed due to the variety of pressure and temperature. Treated by the procedure, 86% of pollen content was spread out, which caused amount increases of vitamin A, vitamin C and cruel lipids. Additional it also caused the decreases of total bacterial count and total coliform group. Furthermore, the results of amplified process showed no significant changes in pollen wall broken rate, A280 and nutrition extraction rate. The newly developed process for disrupting pollen is of high efficiency, high safety, high stability, and easy to be amplified. It has good application prospect.