Abstract: The effects of ultrasonication on heparin extraction were studied, and the technological parameters were optimized. Porcine intestines of commercial slaughtered were treated with ultrasonic assisted salting out to extract heparin. Based on the single factor tests for ultrasonication power, ultrasonication temperature, ultrasonication time, and the ratios of solution to material, a four-variable quadratic orthogonal rotation combination experimental design was applied to optimize the parameters for ultrasonic extraction heparin. The results indicated that the heparin yield was the highest under the conditions which ultrasonication power was set at 300 W, ultrasonication temperature 40℃, ultrasonication time 40 min, and ratio of solution to materials was 15 mL/mg. According to these conditions, the heparin yield was 0.0184%. Which was 10% more than the yield of salting out extraction, and the extraction time was decreased by 2 h. The results suggested that the ultrasonic wave extraction process was feasible, and the extraction yield of heparin was higher than the conventional salting out method, therefore, research on this technology was significant important for practical application.