Abstract: Understanding the effects of acoustic frequency technology (AFT) on the growth of cotton would be helpful to cotton production in Xinjiang Uygur Autonomous Region. An experiment including two different treatments was set in order to investigate the effects of AFT on the growth of cotton. One treatment was to impose the plants with sound wave in special frequencies, the other was control. The results showed that compared to the control sites, by using AFT, the treated plant height of cotton, the width of the fourth expanded leaf from terminal one, boll-bearing branches, number of bolls and single boll weight were increased by 1.71%, 5.25%, 1.14%, 9.22% and 3.34%, respectively. Acoustic waves could increase the treated cotton yield by 11.1%-13.5%, which showed significant difference and average yield increased by 12.7% in the past three years. The effect of treatment was negatively correspondent with the distance between plants and the source of sound. The study show that AFT can both promote vegetative and reproductive growth of cotton, and it can provide a scientific basis for the AFT effects on plant production.