Abstract: In order to explore a new way in aquaculture, a Penaeus vannamei comparative test was conducted by using Biofan in this paper. In one breeding cycle, water environmental factors such as dissolved oxygen, temperature, pH and nitrite, Penaeus vannamei growth and quality and economic efficiency were analyzed. The results show that comparing with aquaculture without biofan, the method improves water dissolved oxygen uniformity, temperature uniformity and increases the dissolved oxygen content of the pond; maintains the water pH value between 7.6-8.9; reduces the nitrite content in water; improves the Penaeus vannamei growth speed and quality; saves 56.9% on power consumption, 15.5% on bait, 45.4% on drug; increases yield by 20.1% per unit area. Test and analysis results showed that using Biofan can improve water environment, reduce aquaculture costs and increase aquaculture production and income, so it has high value of practical application.