Abstract: In order to investigate the effects of different concentrations of bamboo vinegar and bacterial reagent addition on physico-chemical properties of green waste compost, a static aerobic high temperature compost test with an orthogonal experimental design L9(34) was conducted. The variation trends in temperature, pH, EC, total N, P and K concentrations during composting were analyzed, and total N, P, K, Mg, Fe, S concentrations of the compost products in each treatment were compared with analysis of variance (ANOVA) and multiple comparison test. The results showed that the addition of bamboo vinegar and bacterial reagent made the initial temperature rise quickly, reduce pH value and EC value of composting products effectively. The bamboo vinegar and bacterial reagent had very significant effects on total N, P, K, Fe and S concentrations (p<0.01) , which made the total P, K, Fe and S concentrations increase effectively. Furthermore, there existed interaction between bamboo vinegar and bacterial reagent. The total N increase less because of ammonia volatilization, Compared with the control, the addition of bacterial reagent accelerated the N loss, but the addition of a certain concentration of bamboo vinegar can keep more N. The combination of 1:1 000 (v:v) bamboo vinegar of 2 L and bacteria reagent of organic waste of 0.5% has the best effect on green wastes compost.