Abstract: In order to research tree spray target profile probing method, and provide the theoretical support for variable spray based on real-time probing target profile, a tree spray target profile probing platform was improved. The platform could move sensors precisely, and densely probe canopy structure by setting a step as 0.02 m. The platform could record probing data into Access database and showed the results in real time by computer. The platform was used in probing canopy profile of cherry tree in the flowering stage and artificial tree with regular shape. Experiment results indicated that measurement accuracy of regular shape canopy and cherry tree canopy profile probing was 92.8% and 90.0%, respectively, which showed that this method has high accuracy.