Abstract: To change the situation of inefficient, labor intensive, uneven pollination, and low seed quality and yield of hybrid rice seed production when following the traditional rules of male sterile lines and restorer lines planting regularly, a kind of efficient mechanized sorting equipment is urgently needed in the existing engineered mixture sowing seed production. In this paper, experimental research methods were adopted to research the kinematic and structure parameters of the separation tube of sorting machine. The results show that when the length of the male sterile lines is less than 5.8mm, and the restorer lines more than 12mm, the external diameter of separating cylinder is 130mm, the rotation speed of sorting machine is 30r/min, the diameter and depth of the pocket hole is 7mm and 3mm, respectively, then the sorting machine can achieve a good result for seed sorting. This sorting machine can provide basic guarantee for solving seed sorting of male sterile lines and restorer lines of hybrid rice.