Abstract: For the sake of developing new techniques of extraction. High voltage pulsed discharge plasma (HVPDP) for extracting polysaccharide from Auricularia Auricula was studied. Through single factor experiment, there were three factors screened, i.e. pulsed discharge voltage, ratio of material to solvent, and discharge time.the technique conditions were optimized by response surface method, and HVPDP for extracting was discussed. The results showed that the optimal polysaccharides extraction conditions were pulsed discharge voltage 40.3 kV, ratio of material to solvent 40.3, discharge time 4.1 min. Under the conditions, the extraction rate of polysaccharides from Auricularia Auricula was 8.80%. The special feature of HVPDP technique over traditional extraction was short time,low energy consumption and high extraction ratio. The work indicated that extracting polysaccharide from Auricularia Auricula with HVPDP is feasible, and it will provide a base for extracting effective Ingredient with HVPDP.